Lab – Anthro

Reflection Questions

1. Observe each sample for coagulation. Based on what you see…

a. What blood type does Individual #1 have? What is/are the possible genotype(s)

for Individual #1?

b. What blood type does Individual #2 have? What is/are the possible genotype(s)

for Individual #2?

c. What blood type does Individual #3 have? What is/are the possible genotype(s)

for Individual #3?

2. Suppose that the three samples are from two parents and their child.

a. Which individuals are the parents? Which individual is the child? How do you

know?

b. What genotype must each individual have for this scenario to be possible?

c. Draw a Punnett square using the parents’ genotypes. Circle the genotype that

belongs to the child that you have blood typed.

d. What is the percent chance that these two parents would have a child with this

blood type?

3. There is one blood type not represented in these samples.

a. What blood type is it?

b. What would you predict would happen if you added Anti-A serum to this type of

blood?

c. What would you predict would happen if you added Anti-B serum to this type of

blood?

d. Why is the genotype that codes for this blood type considered “codominant”?

Type O blood produces both anti-A and anti-B antibodies, so it will not coagulate with Anti-A or Anti-B Serum.

Lab Kit Materials

Labeled test tubes or small containers:

Individual #1 Blood Sample

Individual #2 Blood Sample

Individual #3 Blood Sample

Anti-A Serum

Anti-B Serum

Five eyedroppers or pipettes

Permanent marker

Six toothpicks or stirrers

Blood Typing Test Plate laminated or placed in a plastic sheet protector

Preparation Steps:

Each student will prepare the following supplies:

Labeled test tubes or small containers (small clear glass will work fine):

Individual #1: Dyed vinegar (this will simulate Type A blood)

Individual #2: Dyed milk (this will simulate Type B blood)

Individual #3: Dyed water (this will simulate Type O blood)

Anti-A Serum: Milk

Anti-B Serum: Vinegar

Five eyedroppers or pipettes

Permanent marker

Six toothpicks or stirrers

Blood Typing Test Plate (attached) laminated or placed in a plastic sheet protector (1 per group)

Student worksheets

Students will stir each sample using a different toothpick for each of the six samples.

Students will observe each sample to see if it has coagulated (agglutinated) or not, and determine the three individuals’ blood types based on the results

Step-by-Step Instructions (Please follow carefully)

Using a different toothpick for each of the six samples, stir each sample.

Observe each sample to see if it has coagulated (agglutinated) or not.

4. Is ABO considered a “Mendelian Trait”?

a. What is “Mendelian genetics”? How would you identify a trait that follows a

pattern of Mendelian genetics? Review the section “Mendelian Genetics” in

Chapter 3 to help you.

b. How does the ABO blood type system follow the rules of Mendelian Inheritance?

c. In what ways is the ABO blood type system more complex than the pea plant

traits that Mendel observed?